Figure 2: RT-PCR analysis of original and alternative transcripts in cerebellum (or whole brain) and testis (or ovary) tissues of human, rhesus, and cynomolgus monkey. (a) Primer location, RT-PCR amplification using primer 1 pair (b) and primer 2 pair (c). GAPDH (120 bp) indicates the positive control. M indicates size marker. AT indicates alternative transcript and NSP is the nonspecific products, confirmed by sequencing.