Performance Characterization and Validation of Saliva as an Alternative Specimen Source for Detecting Hereditary Breast Cancer Mutations by Next Generation Sequencing

Table 2

Quality score, mean depth coverage, and uniformity of the data generated from DNA samples extracted from saliva and their matched blood samples.

Sample ID	Percentage of data Q20	Mean depth of coverage	Uniformity
1601074001Saliva	91.32%	246.8	92.96%
1601074001Blood	91.35%	246.3	98.59%
1601074002Saliva	91.05%	785.8	97.60%
1601074002Blood	91.32%	442.1	97.41%
1601074003Saliva	92.99%	218.2	96.71%
1601074003Blood	92.78%	82.2	98.20%
1601074004Saliva	92.68%	375.2	97.07%
1601074004Blood	93.00%	76.8	94.39%
1601074005Saliva	92.93%	139.9	93.02%
1601074005Blood	92.62%	212.2	96.63%
1601074006Saliva	92.83%	84.5	90.96%
1601074006Blood	92.61%	123.8	98.33%
Mean blood	92.28%	197.2	97.26%
Mean saliva	92.30%	308.4	94.72%