The Power of CRISPR-Cas9-Induced Genome Editing to Speed Up Plant Breeding

Table 1

Useful resources/tools for CRISPR-Cas9 research in plants.

Site	Purpose	Authority
http://www.addgene.org	Access to plasmid resource and tutorial documents [115]	Addgene
https://www.protocols.io	Access to detailed protocol resource	Protocols.io
http://cbi.hzau.edu.cn/cgi-bin/CRISPR	Design optimal sgRNA with 43 plant genomes from Ensembl Plants [24]	Huazhong Agricultural University
http://www.genome.arizona.edu/crispr/CRISPRsearch.html	Predict high specific sgRNA of 8 plant genomes [116]	University of Arizona
http://www.rgenome.net/cas-offinder/	Search for potential off-target sites of sgRNA from 37 plant genomes [117]	Institute for Basic Science, Korea
http://www.e-crisp.org/E-CRISP/index.html	Design sgRNA for genome-libraries projects or individual sequences with 11 plant genomes [118]	German Cancer Research Center (DKFZ)
http://crispr.mit.edu/	Find the CRISPR-Cas9 target sites within an input sequence with Arabidopsis genome [24]	Zhang Lab, MIT
http://chopchop.cbu.uib.no/	Select CRISPR target sites and predict off-target sites with Arabidopsis genome [119]	University of Bergen
http://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design	Design highly active sgRNAs for the provided targets [74]	Broad Institute of MIT and Harvard, Cambridge University
http://eendb.zfgenetics.org/casot/	Open-sourced tool for finding potential off-target sites of any user-specific genome [120]	Peking University
https://groups.google.com/forum/#!forum/crispr	Active discussion groups	Google