Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense

Table 1

Ingredients and compositions of experimental diets (%).

Ingredient	Percentage of dry weight
Casein	30
Fish meal	20
Corn starch	26
Fish oil	4
Soybean oil	2
Vitamin mix	2
Cu-free mineral mix	3
Attractant	3
Cholesterol	0.5
Choline chloride	0.5
Lecithin	0.5
Cellulose	6.5
Sodium carboxymethylcellulose	2
Proximate composition
Crude protein	40.6
Crude lipid	7.47
Crude ash	7.02

Sigma-Aldrich Co., Shanghai, China.
Tecnologica De Alimentos USA.
Xiamen Xinsha Pharmaceutical Co. Ltd., Xiamen, China.
National Golden Dragon Fish Co. Ltd., Shanghai, China.
Vitamin mixture (mg/100 g mixture): vitamin A 420000 IU; vitamin C 6000 mg; α-tocopherol acetate 2000 mg; vitamin D3 120000 IU; vitamin K 1000 mg; vitamin B1 1000 mg; vitamin B2 1000 mg; vitamin B6 1600 mg; vitamin B12 2 mg; niacin 5000 mg; folic acid 400 mg; inositol 6000 mg; biotin 10 mg; and calcium pantothenic 3500 mg, Hangzhou Minsheng Bio-Tech Co., Ltd., China.
Composition of mineral mixture (g/kg diet): KCl 0.84, MgSO₄·7H₂O 3, NaH₂PO₄ 6.45, KH₂PO₄ 3, Ca(H₂PO₄)₂·H₂O 7.95, CaCO₃ 3.15, C₆H₁₀CaO₆·5H₂O 4.95, FeC₆H₅O₇·5H₂O 0.36, ZnSO₄·7H₂O 0.1428, MnSO₄·H₂O 0.0321, Na₂SeO₃ 0.0009, AlCl₃·6H₂O 0.0045, CoCl₂·6H₂O 0.042, and KI 0.0069.
Alanine 0.6%, glycine 0.6%, glutamic acid 0.6%, and betaine 1.2%.
China National Medicine Corporation Co., Ltd., Beijing, China.