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International Journal of Genomics
Volume 2017, Article ID 1746426, 7 pages
https://doi.org/10.1155/2017/1746426
Research Article

Blood Transcriptional Signatures for Disease Progression in a Rat Model of Osteoarthritis

1Department of Molecular Neuropharmacology, Institute of Pharmacology Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland
2Department of Pain Pharmacology, Laboratory of Pain Pathophysiology, Institute of Pharmacology Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland

Correspondence should be addressed to Katarzyna Starowicz; lp.wokark.nap-fi@worats

Received 5 March 2017; Revised 17 May 2017; Accepted 29 May 2017; Published 3 July 2017

Academic Editor: Marco Gerdol

Copyright © 2017 Michał Korostyński et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Biomarkers of osteoarthritis (OA) that can accurately diagnose the disease at the earliest stage would significantly support efforts to develop treatments for prevention and early intervention. We have sought to determine the time course of alterations in peripheral blood gene expression profile associated with the development of OA. Blood samples were collected from a tail vein of individual rats with monosodium iodoacetate- (MIA-) induced OA (2, 14, 21, and 28 days after the treatment). We used whole-genome microarrays to reveal OA-related transcriptional alterations of 72 transcripts. Three main groups of coexpressed genes revealed diverse time-dependent profiles of up- and downregulation. Functional links that connect expression of the gradually downregulated genes to the G13 signaling pathway were indicated. The mRNA abundance levels of the identified transcripts were further analyzed in publicly available gene expression dataset obtained from a GARP study cohort of OA patients. We revealed three-gene signature differentially expressed in both rat and human blood (TNK2, KCTD2, and WDR37). The alterations in expression of the selected transcripts in peripheral blood samples of the patients indicate heterogeneity of the OA profiles potentially related to disease progress and severity of clinical symptoms. Our study identifies several potential stage-specific biomarkers of OA progression.