Characteristics and Validation Techniques for PCA-Based Gene-Expression Signatures

Figure 3

Validation plot for Gender-29 signature applied to Breast dataset. The probesets within the Gender-29 signature are no longer coherent (a), and the ratio of PC1/PC2 is much lower (b). It is also clear that the signature is no longer unique (c). Finally, the biological meaning of the probesets has changed in the Breast dataset (d).

(a)

(b)

(c)

(d)