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International Journal of Genomics
Volume 2017 (2017), Article ID 5149362, 11 pages
Research Article

Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation

1Centre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 Chemin Ste-Foy, Quebec City, QC, Canada G1V 4G5
2Centre de Recherche du CHU de Québec, 2705 Boulevard Laurier, Quebec City, QC, Canada G1V 4G2
3École de Nutrition, Université Laval, 2425 Rue de l’Agriculture, Quebec City, QC, Canada G1V 0A6
4Institut sur la Nutrition et les Aliments Fonctionnels (INAF), 2440 Boulevard Hochelaga, Quebec City, QC, Canada G1V 0A6

Correspondence should be addressed to André Tchernof

Received 29 October 2016; Accepted 29 January 2017; Published 19 March 2017

Academic Editor: Margarita Hadzopoulou-Cladaras

Copyright © 2017 Julie Anne Côté et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. To characterize changes in gene expression profile during human mature adipocyte dedifferentiation in ceiling culture. Methods. Subcutaneous (SC) and omental (OM) adipose tissue samples were obtained from 4 participants paired for age and BMI. Isolated adipocytes were dedifferentiated in ceiling culture. Gene expression analysis at days 0, 4, 7, and 12 of the cultures was performed using Affymetrix Human Gene 2.0 STvi arrays. Hierarchical clustering according to similarity of expression changes was used to identify overrepresented functions. Results. Four clusters gathered genes with similar expression between day 4 to day 7 but decreasing expression from day 7 to day 12. Most of these genes coded for proteins involved in adipocyte functions (LIPE, PLIN1, DGAT2, PNPLA2, ADIPOQ, CEBPA, LPL, FABP4, SCD, INSR, and LEP). Expression of several genes coding for proteins implicated in cellular proliferation and growth or cell cycle increased significantly from day 7 to day 12 (WNT5A, KITLG, and FGF5). Genes coding for extracellular matrix proteins were differentially expressed between days 0, 4, 7, and 12 (COL1A1, COL1A2, and COL6A3, MMP1, and TGFB1). Conclusion. Dedifferentiation is associated with downregulation of transcripts encoding proteins involved in mature adipocyte functions and upregulation of genes involved in matrix remodeling, cellular development, and cell cycle.