The Numerical Predominance and Large Transcriptome Differences of Neutrophils in Peripheral Blood Together Inevitably Account for a Reported Pulmonary Tuberculosis Signature

Table 1

Group comparison^1	PTBsigUp			PTBsigDn
	NES^2	FDR^3	Correlation^3	NES^2	FDR^3	Correlation^3
Neut_PTB versus Neut_HC	3.64	0.000	Positive	−1.97	0.000	Negative
Mono_PTB versus Mono_HC	3.53	0.000	Positive	−1.92	0.000	Negative
CD4_PTB versus CD4_HC	2.93	0.000	Positive	−1.82	0.000	Negative
CD8_PTB versus CD8_HC	2.66	0.000	Positive	−1.71	0.000	Negative

_PTB versus Neut_HC = ranked relative expressions of genes of neutrophils in PTB patients compared to neutrophils in HC donors. Similar explanations apply for the other three group comparisons. ²(+) NES for positive correlation indicating that signature genes were overrepresented at the higher end of a ranked gene list; (−) NES for negative correlation indicating that signature genes were overrepresented at the lower end of a ranked gene list. ³A FDR of 0.05 or lower was regarded as statistically significant for NES (“positive” or “negative”).