MicroRNAs as Biomarkers in Thyroid Carcinoma

Figure 1

miRNA detection workflow. miRNAs can be isolated from different biospecimens. To isolate miRNAs, widely used methods are chemical and column-based techniques. After quantification step, samples are ready for miRNA profiling. Among widely used techniques, there are four established methods: microarray, quantitative PCR (qRT-PCR), massive parallel sequencing (RNA-seq), and digital PCR (dPCR). The sensitivity and specificity are classified as follows: + (low), ++ (moderate), +++ (high). FFPE: formalin-fixed paraffin embedded; FNA: fine-needle aspiration.