The Role of miRNAs as Biomarkers for Pregnancy Outcomes: A Comprehensive Review

Table 4

Studies reporting an association between alteration of miRNA expression and altered BW.

Authors and year	Gestational disorder	Study design	Enrolled population	Samples	Gestational age at sampling (weeks)	Techniques	↑ miRNAs	↓ miRNAs
Song et al., 2013 [18]	LBW	Retrospective	10 cases + 20 controls	Placental tissue + maternal serum	Delivery	qRT-PCR	miR-517a (maternal serum and placental tissue of cases)
Wang et al., 2014 [51]	LGA, IUGR	Retrospective	30 LGA + 30 IUGR + 30 controls	Placental tissue	Delivery	qRT-PCR	mirR-519a (IUGR)	miR-518b (IUGR)
Wang et al., 2014 [51]	LGA	Retrospective	30 LGA + 30 IUGR + 30 controls	Placental tissue	Delivery	qRT-PCR	—	—
Hromadnikova et al., 2017 [26]	IUGR	Retrospective	18 IUGR + 58 controls	Maternal plasma	10	qRT-PCR	—	—
Hu et al., 2014 [16]	Macrosomia	Retrospective	60 cases + 60 controls	Maternal serum	16–20	TLDA − qRT-PCR	—	miR-376a
Li et al., 2015 [52]	Macrosomia	Retrospective	57 cases + 100 controls	Placental tissue + maternal serum	Placenta (delivery) serum (NA)	qRT-PCR	miR-18a, miR-19a, miR-20a, miR-19b, and miR-92a (placental tissue of cases)	miR17, miR18a, miR19a, and miR92a (maternal serum of cases)
Ge et al., 2015 [53]	Macrosomia	Retrospective	35 cases + 20 controls	Maternal serum	18–28	TLDA + qRT-CR	miR-523-3p, miR-3a-3p, and miR-16-5p (cases)	miR-221-3p, miR-143-3p, miR-18a-5p miR-141-3p, and miR200c-3p (cases)
Zhang et al., 2016 [54]	Macrosomia	Retrospective	67 cases + 64 controls	Placental tissue	Delivery	qRT-PCR	miR-21	miR-143
Jiang et al., 2015 [17]	Macrosomia	Retrospective	60 cases + 60 controls	Maternal plasma	16–20 weeks and 1 week from delivery	TLDA + qRT-PCR	—	miR-21
Miura et al., 2015 [55]	Birth weight	Cross-sectional	82 pregnant women	Maternal serum	37-38	qRT-PCR

LBW: low birth weight; qRT-PCR: quantitative real-time polymerase chain reaction; IUGR: intrauterine growth restriction; LGA: large for gestational age; TLDA: TaqMan low-density array.