Overexpression of Chromosome 21 miRNAs May Affect Mitochondrial Function in the Hearts of Down Syndrome Fetuses

Figure 3

End point PCR of SLC25A4/ANT1, DICER, and GAPDH in euploid fibroblasts transfected with a let-7c-5p mimic. (a) Agarose gel of SLC25A4/ANT1, DICER, and GAPDH amplification in: Not transfected cells (lanes 1–3); cells + INTERFERin (lanes 4–6); cells + 5 nM miRNA mimic (lanes 7–9); cells + 10 nM miRNA mimic (lanes 10–12); cells + 25 nM miRNA mimic (lanes 13–15); cells + 50 nM miRNA mimic (lanes 16–18). Amplification products of SLC25A4/ANT1: lanes 1, 4, 7, 10, 13, and 16; amplification products of DICER: lanes 2, 5, 8, 11, 14, and 17; amplification products of GAPDH: lanes 3, 6, 9, 12, 15, and 18. (b) Densitometric analysis of SLC25A4/ANT1 and DICER amplification products obtained by ImageJ software. At 25 nM miRNA mimic concentration, a reduction of target amplification is appreciable.

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