Overexpression of Chromosome 21 miRNAs May Affect Mitochondrial Function in the Hearts of Down Syndrome Fetuses

Figure 5

SLC25A4/ANT1 after let-7c-5p inhibition in trisomic fibroblasts. (a) Transfection of let-7c-5p inhibitor in DS-HFFs induces a significant increase of SLC25A4/ANT1 expression and a decrease of let-7c-5p, as detected by qRT-PCR. Results are expressed as relative mean values ± SEM of three different determination, compared with cells treated only with transfecting agent (set equal to 1). (b) Representative immunoblot of SLC25A4/ANT1 in nontransfected cells (NT), cells transfected only with transfecting agent and cells transfected with 25 nM let-7c-5p inhibitor. Vinculin was used as loading control. (c) Densitometric SLC25A4/ANT1 relative measurement after 72 h treatment. Protein expression was significantly increased after inhibiting treatment if compared with mock transfected cells. The bars show relative mean values ± SEM of two DS-HFFs in duplicate; .

(a)

(b)

(c)