Klhl14-AS isoform expression in adult mouse tissues. Klhl14-AS transcripts were amplified by RT-PCR on total RNA from adult mouse organs. Three different PCR reactions were performed on the same template cDNA. Upper image: A and B isoforms were amplified by using the oligo set 1; middle image: C, D, and E were obtained by using oligo set 2; lower image: Abelson (Abl) amplification was performed as the control (for details see Materials and Methods). Data are representative of three independent experiments.