Research Article

Topological Characterization of Human and Mouse m5C Epitranscriptome Revealed by Bisulfite Sequencing

Figure 5

RNA m5C modification exhibits a clustering effect. (a) Bar graph shows the proportion of clustered m5C sites within 10 nt flanked regions. To evaluate the statistical significance, we generated 1000 permutated results as a comparison with the bars indicating a 99% confidence interval. Using these criteria, m5C methylation showed a strong clustering effect consistently on different RNA families and on different regions of mRNA in human and mouse. Around 50% of the m5C sites were clustered with each other within a 10 bp region. (b) DNA methylation also exhibited a clustering effect. However, the pattern is not that strong when all nucleotide contexts are considered. (c) Line graph showing correlation between RNA/DNA m5C methylation and distance between cytosine sites. RNA m5C methylation showed strong correlation with cytosine sites that are immediately close to each other. The clustering effect of DNA methylation is strong when only CpG context is considered (Figure S4).