Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure Treatment

Table 1

RNA integrity and concentration for all RNA purification replicates in the HHP-treated and control eggs.

Replicate	1		2		3		4		5		All
Homogenization/conservation	Bead mill/RNAlater		Manual/RNAlater		Bead mill/RNAlater		Bead mill/RNAlater		TissueRuptor/dry ice
Group	Control	HHP	Control	HHP	Control	HHP	Control	HHP	Control	HHP	Control	HHP
Mean RIN	8.90	8.70	7.83	8.03	7.97	8.90	8.80	8.87	8.73	8.93	8.45	8.69
RIN SD	0.14	0.20	0.62	0.45	0.50	0.16	0.14	0.42	0.05	0.12	0.58	0.47
Mean concentration (ng/μl)	224.50	318.43	134.80	122.60	175.87	163.13	178.93	187.17	152.37	164.57	173.29	191.18
Concentration SD (ng/μl)	19.77	72.54	11.21	20.66	19.99	76.60	11.42	96.53	18.89	11.43	34.59	93.18
Min RIN	8.70	8.50	7.30	7.50	7.30	8.70	8.70	8.30	8.70	8.80	7.30	7.50
Max RIN	9.00	8.90	8.70	8.60	8.50	9.10	9.00	9.30	8.80	9.10	9.00	9.30
Min conc.	197.40	220.10	119.60	103.10	148.50	59.30	163.70	55.60	126.90	149.70	119.60	55.60
Max conc.	244.00	392.90	146.30	151.20	195.70	241.80	191.20	284.50	172.10	177.50	244.00	392.90