Research Article
Expression Characterization of Six Genes Possibly Involved in Gonad Development for Stellate Sturgeon Individuals (Acipenser stellatus, Pallas 1771)
Table 1
Primers for qPCR analysis.
| | GenBank | Gene | PCR product length (bp) | Primer name | Primer sequence |
| | KX420683 | ar—androgen receptor | 197 | ar-F | 5-CKTGACTCCCCGAACAATCA-3 | | ar-R | 5-AAGGTAGCACGCTGGAACTC-3 |
| | KX420684 | dmrt1—doublesex and mab-3 related transcription factor 1 | 129 | dmrt1-F | 5-CCACCCTGTTCCACTTCCAG-3 | | dmrt1-R | 5-GAAGWGGATGGTGCTGTGCT-3 |
| | KX420685 | sox9—sex-determining region Y-box 9 | 115 | sox9-F | 5-AGGCCGATTCCYCTCACTCT-3 | | sox9-R | 5-TGCAYGTCTGTTTTGGGAGT-3 |
| | KX420686 | foxl2—forkhead box L2 | 120 | foxl2-F | 5-GCCCACCTCGTACAATCCTT-3 | | foxl2-R | 5-CTTAGCTGCTGAGGGTGGTG-3 |
| | KX420678 | cyp17a1—cytochrome P450 family 17 subfamily A polypeptide 1 | 134 | cyp17a1-F | 5-CCGTCGCTTACCTCCTACAC-3 | | cyp17a1-R | 5-CCGTATCGTTGCTTCCAGGT-3 |
| | KX420679 | star—steroidogenic acute regulatory protein | 111 | star-F | 5-AGTACCCTGACCGCCTGTAT-3 | | star-R | 5-TTGTGTCCTGCCCAATCCTC-3 |
| | KX420681 | β-actin | 161 | β-actin-F | 5-TGACCCTGAAGTAYCCMATC-3 | | β-actin-R | 5-CTTCTCTCTGTTRGCYTTGG-3 |
| | KX420682 | gapdh—glyceraldehyde 3-phosphate dehydrogenase | 114 | gapdh-F | 5-AGACACCCGCTCNTCHATCT-3 | | gapdh-R | 5-TCCACGACTCTGTTGCTGTA-3 |
| | KX420680 | 28S rRNA—28S ribosomal RNA | 160 | 28S-F | 5-TGTTTGTGAATGCAGCCCAA-3 | | 28S-R | 5-GACCCCATCCGTTTACCTCT-3 |
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