Table 1: Primers for qPCR analysis.

GenBankGenePCR product length (bp)Primer namePrimer sequence

KX420683arandrogen receptor197ar-F5-CKTGACTCCCCGAACAATCA-3
ar-R5-AAGGTAGCACGCTGGAACTC-3

KX420684dmrt1doublesex and mab-3 related transcription factor 1129dmrt1-F5-CCACCCTGTTCCACTTCCAG-3
dmrt1-R5-GAAGWGGATGGTGCTGTGCT-3

KX420685sox9sex-determining region Y-box 9115sox9-F5-AGGCCGATTCCYCTCACTCT-3
sox9-R5-TGCAYGTCTGTTTTGGGAGT-3

KX420686foxl2forkhead box L2120foxl2-F5-GCCCACCTCGTACAATCCTT-3
foxl2-R5-CTTAGCTGCTGAGGGTGGTG-3

KX420678cyp17a1cytochrome P450 family 17 subfamily A polypeptide 1134cyp17a1-F5-CCGTCGCTTACCTCCTACAC-3
cyp17a1-R5-CCGTATCGTTGCTTCCAGGT-3

KX420679starsteroidogenic acute regulatory protein111star-F5-AGTACCCTGACCGCCTGTAT-3
star-R5-TTGTGTCCTGCCCAATCCTC-3

KX420681β-actin161β-actin-F5-TGACCCTGAAGTAYCCMATC-3
β-actin-R5-CTTCTCTCTGTTRGCYTTGG-3

KX420682gapdh—glyceraldehyde 3-phosphate dehydrogenase114gapdh-F5-AGACACCCGCTCNTCHATCT-3
gapdh-R5-TCCACGACTCTGTTGCTGTA-3

KX42068028S rRNA28S ribosomal RNA16028S-F5-TGTTTGTGAATGCAGCCCAA-3
28S-R5-GACCCCATCCGTTTACCTCT-3