Research Article

Expression Analysis of Nitrogen Metabolism-Related Genes Reveals Differences in Adaptation to Low-Nitrogen Stress between Two Different Barley Cultivars at Seedling Stage

Figure 3

Differential expression of genes related to nitrogen metabolism in roots of the two barley cultivars. Roots were sampled at 0 h, 1 h, 24 h, and 48 h after low-nitrogen stress. Expression is represented as the normalized relative quantity (NRQ) of a target gene’s expression with respect to the two reference genes: HvActin and HvGAPDH. Means and standard errors are shown from the analysis of three biological replicates.