The Maize Corngrass1 miRNA-Regulated Developmental Alterations Are Restored by a Bacterial ADP-Glucose Pyrophosphorylase in Transgenic Tobacco

Figure 2

Transgenic expression of Cg1 and glgC in tobacco. (a) Integration of Cg1 and glgC into the tobacco genome as confirmed by PCR using genomic DNA as template. Primers specific to NPTII, Cg1, or glgC were used. (b) Phenotypes of transgenic (T1) and nontransformed tobacco lines after one month. (c) Phenotypes of transgenic (T0) lines regenerated from transgenic callus and nontransformed tobacco lines after one month. Arrows indicate initiation of flowering in WT, NPTII, and Cg1-glgC lines. (d) Phenotypes of transgenic (T1) and nontransformed tobacco lines obtained from seeds, and Cg1 transgenic (T0) lines regenerated from transgenic callus (right panel) after two months of growth in the soil. Phenotypic alteration is observed in Cg1-expressing lines alone.