Multiple Regression Analysis Reveals MicroRNA Regulatory Networks in Oryza sativa under Drought Stress
(a) Expression level of coregularory miR528-3p/miR812q and their target DWARF-3 by microarray and RT-qPCR. (b) Expression level of coregularory miR395a/miR396g and their target P0651G05.2 by microarray and RT-qPCR. The expression level is expressed as the mean fold changes of 3 replicates. Error bars depict the standard error (). (c) miR528-3p and miR812q cleavage sites on DWARF-3 identified by RLM-5 RACE. (d) miR395a and miR396g cleavage sites on P0651G05.2 identified by RLM-5 RACE. Arrows represent the 5 termini of mRNA fragments, and the numbers beside them denote the cleavage frequency of cloned PCR products at the exact miRNA cleavage sites. The vertical dashes indicate matched RNA base pairs and circles represent GU mismatch whereas no dashes represent other types of mismatch.
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