Table of Contents Author Guidelines Submit a Manuscript
International Journal of Genomics
Volume 2018, Article ID 9469670, 11 pages
https://doi.org/10.1155/2018/9469670
Research Article

Multitarget Effects of Danqi Pill on Global Gene Expression Changes in Myocardial Ischemia

1School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, China
2Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
3School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
4Staidson (Beijing) Biopharmaceuticals Co., Ltd., Beijing 100176, China

Correspondence should be addressed to Chun Li; moc.361@40205891nuhcil and Yong Wang; moc.anis@gnoygnaw_rotcod

Received 16 June 2017; Revised 27 August 2017; Accepted 18 September 2017; Published 1 February 2018

Academic Editor: Martine A. Collart

Copyright © 2018 Qiyan Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Danqi pill (DQP) is a widely prescribed traditional Chinese medicine (TCM) in the treatment of cardiovascular diseases. The objective of this study is to systematically characterize altered gene expression pattern induced by myocardial ischemia (MI) in a rat model and to investigate the effects of DQP on global gene expression. Global mRNA expression was measured. Differentially expressed genes among the sham group, model group, and DQP group were analyzed. The gene ontology enrichment analysis and pathway analysis of differentially expressed genes were carried out. We quantified 10,813 genes. Compared with the sham group, expressions of 339 genes were upregulated and 177 genes were downregulated in the model group. The upregulated genes were enriched in extracellular matrix organization, response to wounding, and defense response pathways. Downregulated genes were enriched in fatty acid metabolism, pyruvate metabolism, PPAR signaling pathways, and so forth. This indicated that energy metabolic disorders occurred in rats with MI. In the DQP group, expressions of genes in the altered pathways were regulated back towards normal levels. DQP reversed expression of 313 of the 516 differentially expressed genes in the model group. This study provides insight into the multitarget mechanism of TCM in the treatment of complex diseases.