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International Journal of Genomics
Volume 2019, Article ID 2839614, 9 pages
Research Article

Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in Age

1National Research Institute of Animal Production, Balice 32-083, Poland
2University Centre of Veterinary Medicine, University of Agriculture in Kraków, Al. Mickiewicza 24/28, 30-059 Kraków, Poland
3Institute of Veterinary Sciences, University of Agriculture in Krakow, 31-120, Poland

Correspondence should be addressed to T. Ząbek; lp.wokark.oozi@kebaz.t

Received 7 February 2019; Accepted 16 April 2019; Published 2 June 2019

Guest Editor: Carrie Finno

Copyright © 2019 T. Ząbek et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Horses are one of the longest-living species of farm animals. Advanced age is often associated with a decrease in body condition, dysfunction of immune system, and late-onset disorders. Due to this, the search for new solutions in the prevention and treatment of pathological conditions of the advanced age of horses is desirable. That is why the identification of aging-related changes in the horse genome is interesting in this respect. In the recent years, the research on aging includes studies of age-related epigenetic effects observed on the DNA methylation level. We applied reduced representation bisulfite sequencing (RRBS) to uncover a range of age DMR sites in genomes of blood leukocytes derived from juvenile and aged horses of native Hucul breed. Genes colocated with age-related differentially methylated regions (age DMRs) are the members of pathways involved in cellular signal transduction, immune response, neurogenesis, differentiation, development, and cancer progression. A positive correlation was found between methylation states and gene expression in particular loci from our data set. Some of described age DMR-linked genes were also reported elsewhere. Obtained results contribute to the knowledge about the molecular basis of aging of equine blood cells.