Molecular Characterization of a Multidrug-Resistant Klebsiella pneumoniae Strain R46 Isolated from a Rabbit

Table 1

Primers used in this study.

Resistance genes	Primer	Sequence (5-3)^b	Restriction endonuclease	Vector	Amplicon size (bp)	Annealing temperature (°C)
catB3^a	P-catB3-F	GGGGTACCACTTACAGGAAACTTGGGGT	Kpn I	pMD™19-T	859	58
	P-catB3-R	CGGGATCCTTAGACGGCAAACTCGAGCC	Bam HI
floR^a	P-floR-F	CGGGATCCGAAGCAAAAGATAATCGGAT	Bam HI	pMD™19-T	1412	54
	P-floR-R	CCAAGCTTTTAGACGACTGGCGACTTCT	Hind III
mdfA2^a	P-mdfA2-F	CGGGATCCTCACATTGCTGAAACATAAACGG	Bam HI	pMD™19-T	1400	55
	P-mdfA2-R	CCAAGCTTCTACCCCTGCTGCGAATTGC	Hind III
qnrB2^a	P-qnrB2-F	CGGGATCCGATTTGACGCATAACCTCAT	Bam HI	pMD™19-T	713	55
	P-qnrB2-R	CCAAGCTTCTAGCCAATAATCGCGATGC	Hind III

^aThe primers for ORFs with the predicted promoter regions. ^bThe underlines present the restriction enzyme sites and their protective bases.