Research Article

Molecular Characterization of a Multidrug-Resistant Klebsiella pneumoniae Strain R46 Isolated from a Rabbit

Table 2

Strains and plasmids used in this study.

Strains and plasmidsRelevant characteristic(s)Reference or source

R46A K. pneumoniae strain named R46 isolated from a rabbitThis study
DH5αEscherichia coli DH5α was used as a host for the recombinant plasmid with the cloned resistance geneOur lab collection
ATCC 25922Escherichia coli ATCC 25922 is an FDA clinical isolateOur lab collection
pMDTM19-T-ORFs/DH5αDH5α carrying the recombinant plasmids pMDTM19-T-ORF (floR, mdfA2, qnrB3, and catB3)This study
E. coli C600E. coli C600 as recipient in the conjugation experiment, RIFrOur lab collection
Plasmids
pMDTM19-TCloning vector for the PCR products of resistance genes of floR, mdfA2, qnrB3, and catB3, AMPrThis study

RIF: rifampin; AMP: ampicillin.