In Vitro Susceptibility and Florfenicol Resistance in Citrobacter Isolates and Whole-Genome Analysis of Multidrug-Resistant Citrobacter freundii

Table 1

Bacteria and plasmids used in this work.

Strain or plasmid	Relevant characteristic(s)^a	Reference or source
Strain
25 Citrobacter strains (Table S1)	25 Citrobacter strains from 405 nonduplicated bacterial isolates	This study
DH5α	Escherichia coli DH5α was used as a host for the cloned resistance genes	Our laboratory collection
ATCC25922	Escherichia coli ATCC25922 was used as the quality control for antimicrobial susceptibility testing	Our laboratory collection
pUCP24-ORFs/DH5α	DH5α carrying the recombinant plasmids of pUCP24 cloned with resistance gene ORFs with their upstream promoter regions (floR, blaCMY-97, blaDHA-1, blaSHV-12, blaTEM-1b, qnrB4, and qnrB6)	This study
pUCP20-ORFs/DH5α	DH5α carrying the recombinant plasmids of pUCP20 cloned with resistance gene ORFs with their upstream promoter regions (catA2, strA, strB, aac6, aac3, aacA4cr, and aac(6)-IIc)	This study
EC600	Escherichia coli C600 was used as the recipient for conjugation experiment; RIF^r	Our laboratory collection
Plasmid
pUCP20	Cloning vector for the PCR products of resistance gene ORFs with the promoter regions; AMP^r	Our laboratory collection
pUCP24	Cloning vector for the PCR products of resistance gene ORFs with the promoter regions; GEN^r	Our laboratory collection

^aORFs: open reading frames; r: resistance; RIF: rifampin; AMP: ampicillin; GEN: gentamicin.