Review Article

The Potential Use of the CRISPR-Cas System for HIV-1 Gene Therapy

Figure 2

Principle of the CRISPR-Cas technology. A guide RNA (green) is composed of a crRNA sequence that is specific to a target DNA (light-blue), linked to a tracrRNA sequence that interacts with Cas endonuclease. The complex recognizes the PAM sequence (protospacer adjacent motif), a trinucleotide immediately following the targeted DNA to orientate its cleavage. Genomic DNA is showed in dark blue.