Florfenicol Resistance in Enterobacteriaceae and Whole-Genome Sequence Analysis of Florfenicol-Resistant Leclercia adecarboxylata Strain R25

Table 1

Bacterial strains and plasmids used in this study.

Strains and plasmids	Description	Source
Strain
L. adecarboxylata R25	Multiresistant isolate derived from rabbits	This study
E. coli DH5α	Used as a host for cloning of PCR products	Our lab collection
E. coli ATCC25922	Used as a control strain	Our lab collection
pMD™19-T-ORFs/E. coli DH5α	E. coli DH5α carrying the recombinant plasmid pMD™19-T carrying resistance gene ORFs and promoter regions (aac(6)-Ib-cr, aadA16, arr-3, qnrB6, and floR)	This study
Plasmid
pMD™19-T	Cloning vector for the PCR products of all resistance genes and its promoter region, Amp^r	This study

Abbreviations: Amp: ampicillin; r: resistance.