Genetic Diversity of Orobanche crenata Populations in Ethiopia Using Microsatellite Markers

Table 1

List of transferred SSR markers used to test 96 O. crenata samples with major allele frequency, number of alleles, gene diversity, heterozygosity, and PIC values.

Locus	SSR sequence	Forward primer sequence (5-3)	Reverse primer sequence (5-3)	Average Tm (°C)	Expected size (bp)	Number of allele	Major allele frequency	Gene diversity	Heterozygosity	PIC value
Ocum-003	(GAT)8	CAAAGATGGTGGTTTTGCG	CTCGAACGCAAACTTTTGAA	55	94	7	0.4	0.77	0.72	0.75
Ocum-006	(CT)8	CTTATGTATGTTGTTCTTCTCTGCC	CATACATCCAATTAACATACAAGCA	57	90	7	0.34	0.8	0.63	0.77
Ocum-011	(CA)8	GCCGTGAACTCCACTACCAC	GAGTTAGGGTCAGTCTTGCGA	60	274	13	0.22	0.89	0.44	0.87
Ocum-023	(AG)9	CATCACCTCGAGTTTTCCGT	CGCAAGTTCACGAATTGAA	55	157	13	0.35	0.83	0.35	0.82
Ocum-043	(AGG)10	AGGTGCACTTAACCTTGACCTT	CTGCAGGTGGTCATGCTAGA	59	104	11	0.15	0.89	0.4	0.87
Ocum-052	(AG)10	CATGTCTAAGCTTTTGGCTCG	CAAGACTTGGAACAAGCAAATC	57	108	7	0.21	0.83	0.42	0.8
Ocum-059	(TC)11	TCTTGATTTGTATATGTCTGATGCAAT	ATGCTACAATAGAAATACACAACGAAC	56	90	17	0.13	0.93	0.43	0.92
Ocum-063	(AG)11	AACCAAGTTGATGCATCCGT	TCCCTCGGCATTCAGACTTA	57	90	4	0.53	0.64	0	0.59
Ocum-075	(CA)12	TGTGGATAGAGTATAAGCTACCAGTTC	TTCCCGTAGCTTGGAGAATG	60	110	10	0.22	0.85	0.32	0.82
Ocum-081	(CA)13	TTACAAGGTGAAACCACCCA	CAGCTACTGTCCGCAAGAAA	56	90	11	0.16	0.89	0.47	0.87
Ocum-094	(GT)15	TGGGAGCTTTGTACAGACACTG	GTTTTCTATTAAACCGTAACAAACTCT	58	141	6	0.33	0.77	0	0.73