circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8

Figure 5

miR-34a-5p targeted TNFAIP8 in NSCLC cells. (a) miR-34a-5p binding sites on 3UTR of TNFAIP8 were presented. (b, c) Dual-luciferase reporter assay was used to analyze the association between miR-34a-5p and TNFAIP8. (d, e) The expression level of TNFAIP8 was assessed by western blot assay in A549/DDP and H1299/DDP cells transfected with miR-NC, miR-34a-5p, anti-miR-NC, or anti-miR-34a-5p. (f–h) RT-qPCR and western blot assays were used to assess the expression levels of TNFAIP8 in NSCLC tissues and cells. (i, j) Pearson’s correlation analysis was used to assess correlation between TNFAIP8 and miR-34a-5p or circHUWE1. (k) The expression level of TNFAIP8 was examined by western blot in A549/DDP and H1299/DDP cells transfected with sh-NC, sh-circHUWE1, sh-circHUWE1+anti-miR-NC, or sh-circHUWE1+anti-miR-34a-5p. .

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