circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8

Figure 6

The upregulation of miR-34a-5p-induced effects on NSCLC cells were abolished by TNFAIP8 overexpression. (a) The overexpression efficiency of TNFAIP8 was checked by RT-qPCR. (b–q) A549/DDP and H1299/DDP cells were transfected with miR-NC, miR-34a-5p, miR-34a-5p+pcDNA, or miR-34a-5p+TNFAIP8. (b) Western blot was conducted to assess TNFAIP8 level in A549/DDP and H1299/DDP cells. (c–i) IC₅₀ of DDP and proliferation of A549/DDP and H1299/DDP cells were assessed by colony forming and CCK-8 assays. (j–l) Cell apoptosis and cell cycle distribution were evaluated by flow cytometry. (m, n) Transwell assay applied to measure migration and invasion of A549/DDP and H1299/DDP cells. (o–q) The levels of PCNA, c-caspase 3, and MMP 13 were quantified by western blot assay in A549/DDP and H1299/DDP cells. .

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