Hepatitis C Virus E2 Protein Ectodomain Is Essential for Assembly of Infectious Virions

<table>Infectivity of transcomplemented JFH1<svg height="11.425" id="M57" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.025 11.425" width="11.025" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.363)"><path d="M600 0h-557v24l268 633l28 8l261 -641v-24zM497 50l-194 489l-196 -489h390z" id="x394"></path></g>
</svg>E1E2 and JFH1/Con1E2 defective viral genomes. <i>In vitro</i> transcribed genomic JFH1ΔE1E2, Con1E2 defective RNAs and the full-length JFH1 genome were delivered to S6.1/E1E2:2a (a) and S6.1/E1E2:1b (b) packaging cell lines that stably express E1 and E2 HCV proteins of genotype 2a and 1b, respectively. From day 3 to day 7 after transfection, cell supernatants were collected and tested for infectivity (pfu/ml) by serial dilution and immunofluorescence against core protein.</table>

International Journal of Hepatology

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Figure 7

Figure 7: Hepatitis C Virus E2 Protein Ectodomain Is Essential for Assembly of Infectious Virions 