Fractalkine and Other Chemokines in Primary Biliary Cirrhosis

Figure 3

Autologous liver mononuclear cells adhesion assay using endothelial cells and BEC after stimulation with TLR4 ligand (LPS). Adherent liver mononuclear cells were stained and counted in ten random high-power microscopy fields. Liver mononuclear cells from PBC livers adhered in greater numbers than did liver mononuclear cells from controls using either endothelial cells or biliary epithelial cells, whereas liver mononuclear cells adhered only minimally to liver sinusoidal endothelial cells in all instances. Other TLR ligands did not accelerate liver mononuclear cells adhesion with neither endothelial cells nor biliary epithelial cells (data not shown).