Characterization of Autoantibodies against the E1 Subunit of Branched-Chain 2-Oxoacid Dehydrogenase in Patients with Primary Biliary Cirrhosis
Figure 3
Three-dimensional mapping of the epitopes of BCOADC-E1. (a) Structural visualization of the major determinant (epitope 6) of BCOADC-E1. A three-dimensional structure of the E1 heterotetramer [21] is shown with the E1 subunit in green and the E1 subunit in cyan. The major determinant of E1 is shown in red, with stick representations of the side chains of amino acids 137–142 and 153–156. (b) The major determinant overlaps with the active center pocket of E1 [21]. The major determinant (epitope 6, aa 134–168) is shown in red, while the residues in the active site are shown in magenta with stick representation. (c) Surface representations of the epitopes of E1. The epitopes on the molecular surface of E1 are drawn with the epitope numbers. The strengths of the antibody reactivities are indicated in red and pink, which correspond to +++ and ++ in Table 3, respectively.
