Figure 7: Activation of BMPR-I. Bone morphogenetic protein receptor type I (BMPR-I) was immunoprecipitated from the liver lysates of mice fed with regular (control) or ethanol-containing (alcohol) Lieber-De Carli liquid diets, and of mice fed with diets containing 0.2% (normal) or 2% (overload) carbonyl iron, as described in Materials and Methods. The phosphorylation of BMPR-I on serine residues in the immune complexes was determined by western blotting employing an anti-phosphoserine antibody (a), and the total levels of BMPR-I protein were detected by western blotting with an anti-BMPR-I antibody (b). (c) Autoradiographs from different experiments ( ) were scanned by a densitometer, and phospho-BMPR-I (p-BMPR-I) expression in each sample was quantified by normalizing to immunoprecipitated BMPR-I protein level. Normalized p-BMPR-I expression in alcohol or high iron-fed mice was expressed as fold expression of that in the control mice or in mice fed with normal iron diet.