Combined Stimulation with the Tumor Necrosis Factor
and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices
Figure 6
Expression of cyclin D1, Cdk1, and ERK1/2. Northern blot analysis of cyclin D1 and Cdk1 (a) in liver (NL), core (c), freshly prepared slices (Sl), after preincubation (0), and at different times of culture in absence (−EGF) or presence (+EGF) of EGF. Regenerating liver, 24 h after partial hepatectomy (PH24), was used as a positive control of proliferation. Hybridization of 28S ribosomal RNAs was used to control equal loading of RNAs in each lane. Western blot analysis of phospho-(P-)ERK1/2, total ERK1/2, cyclin D1, and Cdk1 (b) in cultured slices at the indicated times of culture in absence (−EGF) or presence (+EGF) of EGF and/or TNFα. Primary cultures of isolated hepatocytes stimulated by EGF (at 48 and 72 h) were used as control of proliferation.
