International Journal of Hepatology / 2012 / Article / Fig 2

Research Article

Cyanamide Potentiates the Ethanol-Induced Impairment of Receptor-Mediated Endocytosis in a Recombinant Hepatic Cell Line Expressing Alcohol Dehydrogenase Activity

Figure 2

Kinetics of internalization (a) and degradation (b) of 125I-ASOR in HAD cells after 7 days of treatment with 25 mM ethanol. Cells were treated with (□) or without (■) ethanol for 7 days as previously described. At the end of the treatment period, cells were incubated with Eagles medium containing 1% BSA and 2 ug per mL iodinated ASOR. At the indicated times, duplicate plates were removed from the 37°C incubator, the cells were removed from the plates with 2 mM EDTA, and internalization (pellet associated, a) and degradation (acid-soluble media radioactivity, b) were determined. Data are presented as fmoles internalized or degraded per million cells and are means ± SEM for 6–10 experiments. Values significantly different from controls are indicated, .

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