Research Article

Lipid Droplet Accumulation and Impaired Fat Efflux in Polarized Hepatic Cells: Consequences of Ethanol Metabolism

Figure 7

Ethanol and oleate-induced apoptosis in polarized hepatoma cultures. WIF-B cells were cultured for 48 hours in the absence (control media, C) or presence of 25 mM ethanol (E25) with or without the presence of 0.5 mM oleic acid (O and E25 + O). Apoptotic cell death was measured by spectrofluorometric detection of caspase-3 activity. Results are expressed as nanomoles of detected fluorometric substrate (AMC) cleaved and released by active caspase-3 enzyme per mg protein for four independent experiments. *Significantly different ( ) from control group.
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