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International Journal of Hepatology
Volume 2013, Article ID 103830, 10 pages
http://dx.doi.org/10.1155/2013/103830
Research Article

Enhanced Antitumor Activity with Combining Effect of mTOR Inhibition and Microtubule Stabilization in Hepatocellular Carcinoma

1Department of Clinical Oncology, The Chinese University of Hong Kong, Hong Kong
2Cancer Drug Testing Unit, State Key Laboratory of Oncology in South China, Hong Kong Cancer Institute and Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong
3Department of Otolaryngology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA

Received 20 November 2012; Accepted 4 January 2013

Academic Editor: Bin Lu

Copyright © 2013 Qian Zhou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: (a) Everolimus treatment induced Akt phosphorylation in HCC cells. HepG2, Hep3B and SNU398 Cells (3×105) were treated with 0.1μM everolimus or DMSO control for 48 hrs and 72 hrs. The expression levels of pi-Akt (Ser473), Akt and actin were assessed by Western blotting. Similar results were observed in 3 independent experiments. (b) Everolimus/patupilone combination did not suppress Akt phosphorylation in HCC cells. HepG2, Hep3B and SNU398 cells (3×105) were treated with everolimus (0.1 μM) and/or patupilone (Pat) (0.5nM) for 24 hrs. The expression levels of pi-Akt (Ser473), Akt and actin were assessed by Western blotting. Similar results were observed in 3 independent experiments.

  1. Supplementary Figure