Demonstration of Hepatitis C Virus RNA with In Situ Hybridization Employing a Locked Nucleic Acid Probe in Humanized Liver of Infected Chimeric Mice and in Needle-Biopsied Human Liver
Figure 2
Confirmation of the specificity of LNA-based ISH. (a) HE stain, (b) ISH after DNase treatment, (c) ISH after RNase treatment, (*) mouse hepatocytes, and (**) human hepatocytes. The mouse hepatocytes are densely eosinophilic, while the human hepatocytes appear pale with deposition of fat droplets. HCV-RNA is observed only in the human hepatocytes in the chimeric liver infected with HCV genotype 1b. The HCV-RNA signals are resistant to DNase treatment but disappear after RNase treatment.
