A Combination of Leucine, Metformin, and Sildenafil Treats Nonalcoholic Fatty Liver Disease and Steatohepatitis in Mice
Sild-Met-Leu effects on liver histology in mice. Mice were fed a low-fat (LF) diet or high-fat atherogenic (HC) diet for 6 weeks and then randomized to the indicated treatment groups for an additional 6 weeks. (a) Liver sections were stained with hematoxylin and eosin (H&E) at the end of the study. Representative images for each group are shown. (b) PPAR alpha gene expression in liver extracts was measured and expressed as mean ± SEM of fold change of control (). Sild: sildenafil, Met: metformin, Leu: leucine.