Figure 7: Immunohistochemistry for CGRP. (a) Representative microphotographs of CGRP-immunoreactive fibers surrounding mesenteric arteries in myenteric plexus preparations of OVX female rats. Daidzein (10 mg/kg or 20 mg/kg p.o.) or daidzein plus fulvestrant (20 mg/kg p.o. and 2.5 mg/kg s.c., resp.) were administered daily during 3 days. The photomicrographs were captured at 400x magnification; the scale bar indicates 50 μm. (b) Bars represent the mean ± SEM ( ) of relative morphometric units measured as stained area/total area. Specific immunoreactivity in every tissue was calculated as the difference between anti-CGRP-incubated and nonprimary antibody-incubated samples. between daidzein-treated (20 mg/kg) and either daidzein plus fulvestrant-treated or untreated OVX rats.