Heme Oxygenase-1 Induction and Organic Nitrate Therapy: Beneficial Effects on Endothelial Dysfunction, Nitrate Tolerance, and Vascular Oxidative Stress
Figure 8
Post-transcriptional regulation of the human HO-1 expression. Human endothelial EA.hy 926 cells were transiently transfected with pGL3-Control-HO-1-3-UTR and pRenilla (normalization of transfection efficiency). After 24h the cells were treated with 50 μM nitroglycerin (GTN) or PETN (or the solvents ethanol [EtOH] or DMSO) for 6h. Extracts were prepared and luciferase and Renilla activity were determined. The luciferase activity was normalized to the renilla activity. Shown (mean ± SEM; ) are the normalized luciferase activity values. The normalized luciferase activity of the cell treated with solvent were set to 100% (*= ; ns = not significant versus EtOH- or. DMSO treated cells).
