High Blood Pressure Effects on the Blood to Cerebrospinal Fluid Barrier and Cerebrospinal Fluid Protein Composition: A Two-Dimensional Electrophoresis Study in Spontaneously Hypertensive Rats

Table 1

List of proteins detected in CSF that are altered, high or low in SHR compared to WKY using 2D electrophoresis and identified by mass spectrometry as described in Section 2.

ID	Protein	NCBI nr	Theoretical MW (Da)/pI	Matched peptide	Sequence coverage (%)	Mascot score	Missed cleavage	Fold SHR expression
1a	𝛼-1-Antitrypsin	gi:112889	46.278/5.70	8	22	82	1	+1.75
1b	𝛼-1-Antitrypsin	gi:112889	46.278/5.70	9	27	81	1	+2.95
1c	𝛼-1-Antitrypsin	gi:112889	46.278/5.70	9	25	83	1	+2.60
2a	Apolipoprotein A1	gi:178775	28.070/5.27	*Identification from Finehout et al., 2004 [12]				+5.37
2b	Apolipoprotein A1	gi:178775	28.070/5.27	*Identification from Finehout et al., 2004 [12]				+5.79
3	Apolipoprotein E	gi:1703338	35.788/5.23	9	27	78	0	−1.92
4a	Transthyretin	gi:136467	15.824/5.77	5	43	97	1	−2.86
4b	Transthyretin	gi:136467	15.824/5.77	7	59	85	1	−3.54
5	Albumin	gi:55391508	70.682/6.09	13	25	190	1	+1.41
6	𝛼-2-HS Glycoprotein	gi:231468	38.757/6.05	5	21	85	0	−1.32
7	Transferrin	gi:122066515	78.512/7.14	12	22	169	1	−2.04
8	𝛼-1-𝛽 Glycoprotein	gi:23503038	51.870/5.51	*Identification from Finehout et al., 2004 [12]				−3.26
9	Ig gamma-2A chain C region	gi:121052	35.677/7.72	3	11	59	1	+1.79
10	Kininogen	gi:60293582	48.828/6.08	6	16	63	1	−1.52
11a	Vitamin D Binding Protein	gi:139643	55.106/5.65	14	34	166	1	+2.21
11b	Vitamin D Binding Protein	gi:139643	55.106/5.65	8	21	55	1	+1.24
12a	Haptoglobin	gi:59808182	39.052/6.10	8	22	89	1	+2.67
12b	Haptoglobin	gi:59808182	39.052/6.10	5	11	63	0	+3.42
13	Carbonic anhydrase II	gi:9506445	29.698/6.9	6	33	121	0	−2.83
14	Alpha-1-macroglobulin	gi:205384	168.422/6.46	10	8	71	1	+1.83