International Journal of Hypertension / 2013 / Article / Fig 2

Review Article

AT2 Receptor-Interacting Proteins ATIPs in the Brain

Figure 2

Interaction between ATIP1 and wild-type or mutated AT2 receptors. The C-terminus domain of ATIP1 (ATIP) interacts with the C-terminal region of human AT2 receptor, either wild-type (WT) or mutated (R324Q, I337V). The yeast reporter strain HF7 expressing the pairs of indicated hybrid proteins was analyzed for histidine auxotrophy and β-galactosidase expression as described [14]. Transformants were plated on medium with histidine (left), without histidine (His, middle), or replica-plated on Whatman filters and tested for β-galactosidase activity (βGal, right). Growth in the absence of histidine and blue color in the β-galactosidase assay indicate interaction between hybrid proteins (WT: wild-type sequence; Ctrl; empty vector).