Table 1: Klotho expression in the aorta.

Author Species Klotho expression Major findings Effect of FGF23 on vascular calcification References

Mitani Rat Negative Klotho was expressed in the kidney but not in aorta or heart. Not evaluated [69]
Nakano-KurimotoHuman Positive (mRNA) Klotho was expressed in human coronary artery smooth muscle cells but not in endothelial cells. Not evaluated [70]
Donate-Correa Human Positive (mRNA) Klotho was expressed in human thoracic aorta and thrombus material from patients with acute coronary syndrome. Not evaluated [71]
Lim Human Positive Klotho was expressed in human aorta or human aortic smooth muscle cells. Calcitriol restored the Klotho expression, decreased by uremic toxin.FGF23 had an anticalcification effect in the presence of calcitriol. [72]
SciallaHuman Negative FGF23 and Klotho were not detected in human or mouse VSMCs.FGF23 had no effect on vascular calcification in human vascular smooth muscle cells or mouse aortic rings.[73]
Lindberg Mouse Negative Klotho expression was undetectable by immunohistochemistry and Western blot analysis. FGF23 had no effect on vascular calcification in bovine vascular smooth muscle cells. [74]
Fang Mouse (ldlr−/−) Positive Early CKD reduced vascular Klotho and FGF23 expression. Not evaluated [75]
Jimbo Rat Positive Klotho expression was detected in the aorta of normal and uremic rats. FGF23 accelerated vascular calcification in Klotho-overexpressed rat VSMCs and rat aortic rings. [76]