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International Journal of Hypertension
Volume 2014, Article ID 735627, 7 pages
Research Article

Role of Renal Nerves in the Treatment of Renovascular Hypertensive Rats with L-Arginine

1Department of Physiological Sciences, Health Sciences Center, Federal University of Espírito Santo, Avenida Marechal Campos 1456, 29040-577 Vitória, ES, Brazil
2Department of Morphology, Health Sciences Center, Federal University of Espirito Santo, Vitoria, ES, Brazil
3Faculdade Brasileira Medical School, Vitoria, ES, Brazil
4EMESCAM Medical School, Vitoria, ES, Brazil

Received 22 June 2014; Revised 24 August 2014; Accepted 9 September 2014; Published 30 September 2014

Academic Editor: Markus Schlaich

Copyright © 2014 Sonia Alves Gouvea et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The purpose was to determine the role of renal nerves in mediating the effects of antihypertensive treatment with L-arginine in a renovascular hypertension model. The 2K1C (two-kidney one-clip model) hypertensive rats were submitted to bilateral surgical-pharmacological renal denervation. The animals were subdivided into six experimental groups: normotensive control rats (SHAM), 2K1C rats, 2K1C rats treated with L-arginine (2K1C + L-arg), denervated normotensive (DN) rats, denervated 2K1C (2K1C + DN) rats, and denervated 2K1C + L-arg (2K1C + DN + L-arg) rats. Arterial blood pressure, water intake, urine volume, and sodium excretion were measured. The 2K1C rats exhibited an increase in the mean arterial pressure (MAP) (from 106 ± 3 to 183 ± 5.8 mmHg, ), whereas L-arg treatment induced a reduction in the MAP (143 ± 3.4 mmHg) without lowering it to the control level. Renal nerve denervation reduced the MAP to normotensive levels in 2K1C rats with or without chronic L-arg treatment. L-arg and denervation induced increases in water intake and urine volume, and L-arg caused a significant natriuretic effect. Our results suggest that renal sympathetic activity participates in the genesis and the maintenance of the hypertension and also demonstrate that treatment with L-arg alone is incapable of normalizing the MAP and that the effect of such treatment is not additive with the effect of kidney denervation.