Excess of Aminopeptidase A in the Brain Elevates Blood Pressure via the Angiotensin II Type 1 and Bradykinin B2 Receptors without Dipsogenic Effect

Figure 1

Immunoreactivity of the aminopeptidase A (APA) in the medulla oblongata of Wistar-Kyoto rats. Sections were immunostained with anti-APA antibody as described in the Materials and Methods. (a) The low-magnification photograph of medulla oblongata. APA immunoreactivity is diffusely distributed. The square in the photo indicates the area shown in (b) and (c). (b) Negative control. (c) APA immunoreactivities were observed in ambiguous nucleus (Amb) and rostral ventrolateral medulla (RVLM). (d) Higher magnification of APA-stained neuron. Neurons and neuropils were positive for APA immunoreactivity. The scale bar denotes 200 m in (a) and (b) and 20 m in (c).

(a)

(b)

(c)

(d)