A Strong Impact of Genetic Background on Gut Microflora in Mice
Figure 6
ARISA analysis of DNA from cecal contents of 129 N5. This DKO colony was established by backcrossing the mixed strain B6;129 colony to 129Sv/J for 5 generations. The plots were generated from 15 DKO mice on AIN diet (AINDKO), 18 DKO mice on LabDiet (LabDietDKO), 7 DKO mice on AIN diet supplemented with 1% yeast (Yeast DKO), 13 non-DKO mice on AIN diet (AIN non-DKO), and 13 non-DKO mice on LabDiet (LabDiet non-DKO). Non-DKO mice were the sibs of DKO mice, heterozygous for Gpx1 and/or Gpx2 (thus no inflammation). DKO mice on LabDiet did not have the distinctive peaks at 385–390 bp, 421 bp, 481 bp, and 498 bp found in other groups. DKO mice on the yeast-containing diet had a similar profile as AIN-fed mice, except having a prominent 317 bp peak. The 442 bp ribotype found in these 129 N5 DKO mice had lower abundance in the non-DKO sibs on both LabDiet and AIN diets.
