Cytotoxicity and Induction of Inflammation by Pepsin in Acid in Bronchial Epithelial Cells
Figure 1
Exposure of cells for 5 minutes induced more cytotoxicity as measured by LDH after 20 hours incubation at pH 2.0 (mean (SEM) 57% ± 5.77%) compared to both pH 1.5 and pH 2.5 (22% ± 2.17%, and 28% ± 2.81%, resp., both ). LDH release was pH-dependent (). There was a trend for a pepsin effect (), with lower LDH release with pepsin at all concentrations compared to no pepsin, independent of pH levels.
