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International Journal of Inflammation
Volume 2013, Article ID 816283, 8 pages
Research Article

The Effects of Insufflation Conditions on Rat Mesothelium

1Research Centre for the Molecular Basis of Disease, Griffith Health Institute, Griffith University, Gold Coast, QLD 4222, Australia
2School of Pharmacy and Medical Science, University of South Australia, Adelaide, SA 5001, Australia
3Fisher & Paykel Healthcare Limited, 15 Maurice Paykel Road, East Tamaki, Auckland 2013, New Zealand
4Graduate School of Medicine and Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW 2500, Australia

Received 27 March 2013; Revised 11 June 2013; Accepted 11 June 2013

Academic Editor: G. Rogler

Copyright © 2013 Andrew K. Davey et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aim. The aim of this investigation was to examine the alterations in the peritoneum after cold dry CO2, heated dry CO2, and humidified heated CO2 at pressures equivalent to intraperitoneal pressures used in human laparoscopy. Methods. Eighteen rats were divided into 4 treatment groups—group 1: untreated control; group 2: insufflation with cold dry CO2; group 3: insufflation with heated, dry CO2; group 4: insufflation with heated and humidified CO2. The abdomen was insufflated to 5 mm/Hg (flow rate 50 mL/min) for 2 h. Twelve hours later, tissue samples were collected for analysis by light microscopy (LM) and scanning electron microscopy (SEM). Results. Group 1: no abnormalities were detected. Group 2: specimens revealed an inflammatory response with loss of mesothelium and mesothelial cell nuclei showing lytic change. Cells were rounded with some areas of cell flattening and separation. Group 3: some animals showed little or no alteration, while others had a mild inflammatory response. Mesothelial cells were rounded and showed crenation on the exposed surface. Group 4: specimens showed little change from the control group. Conclusions. The LM results indicate that insufflations with heated, humidified CO2 are the least likely to induce mesothelial damage.