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International Journal of Inflammation
Volume 2014 (2014), Article ID 580320, 7 pages
http://dx.doi.org/10.1155/2014/580320
Research Article

Prophylactic Antioxidant Potential of Gallic Acid in Murine Model of Sepsis

1Siddhartha Institute of Pharmacy, Near I.T. Park, Sahastradhara Road, Dehradun, India
2City Heart Centre, 19 Cross Road, Dehradun 248001, India
3Nova College Pharmacy, Vegavaram, Jangareddigudem, Andhra Pradesh 534447, India

Received 12 February 2014; Revised 11 May 2014; Accepted 12 May 2014; Published 11 June 2014

Academic Editor: Madhav Bhatia

Copyright © 2014 Harikesh Maurya et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Present study is to investigate the effect of Gallic acid pretreatment on survival of septic animals and oxidative stress in different organs like lungs, liver, kidney, spleen, and vascular dysfunction of mice. Sepsis was induced by cecal ligation and puncture (CLP) in healthy adult male albino mice (25–30 g) and was divided into 3 groups each consisting of 6 animals, that is, sham-operated (SO group (Group I), SO + sepsis (Group II), and Gallic acid + sepsis (Group III)). Group III animals were pretreated with Gallic acid at the dose rate of 20 mg/kg body weight for 2 days before induction of sepsis. Animals were sacrificed on 8th day and the tissue samples were obtained for further investigation on lipid peroxidation (LPO), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GSH). Gallic acid pretreatment significant () reduces kidney, spleen, liver, and lungs’ malondialdehyde level in septic mice. However, it fails to improve reduced glutathione level in all given organs, while, Gallic acid pretreated mice showed significant improvement in SOD activity of kidney and spleen when compared to septic mice. Finally, the beneficial effects of Gallic acid pretreatment in sepsis are evident from the observations that Gallic acid partially restored SOD and catalase activity and completely reversed lipid peroxidation. Further studies are required to find out the possible mechanisms underlying the beneficial effects of Gallic acid on large population.